β-glucan-containing polysaccharide extract from the grey oyster mushroom [Pleurotus sajor-caju (Fr.) Sing.] stimulates glucose uptake by the L6 myotubes
نویسندگان
چکیده
Mushroom β-glucan has the ability to affect many cellular functions, including cellular glucose uptake. Although cumulative evidence in literature suggests a connection between β-glucan and reduction of blood glucose concentration, a mechanism of how β-glucan affects cellular glucose uptake has not been demonstrated. In this study, we analyzed the effect of β-glucan containing polysaccharide extract from mushrooms on glucose uptake by the L6 myotubes. We extracted crude polysaccharide from fruiting bodies of the grey oyster mushroom (Pleurotus sajor-caju), using hot water and protein removed by the Sevag reagent. The presence of β-glucan in the extract was confirmed by FT-IR analysis. We found that the partially purified polysaccharide extract from the grey oyster mushroom stimulated glucose uptake by the rat L6 muscle cells. In addition, laminarin, a small soluble β-glucan, enhanced the cellular glucose uptake. Results from this study suggest that β-glucan in the extract might promote the effect to the cells. The effect of purified mushroom β-glucan on the L6 cells requires additional analyses, and mechanistically how the mushroom β-glucan affects blood glucose concentration becomes our future goal.
منابع مشابه
AMP-activated protein kinase mediates insulin-like and lipo-mobilising effects of β-glucan-rich polysaccharides isolated from Pleurotus sajor-caju (Fr.), Singer mushroom, in 3T3-L1 cells.
Mushrooms have been used to treat various diseases for thousands of years. In the present study, the effects of Pleurotus sajor-caju mushroom on lipogenesis, lipolysis and oxidative stress in 3T3-L1 cells were investigated. The β-glucan-rich polysaccharides (GE) from P. sajor-caju stimulated lipogenesis and lipolysis but attenuated protein carbonyl and lipid hydroperoxide levels in 3T3-L1 cells...
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